| 货号 | 6309S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM RSK1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). |
| 存放说明 | -20C |
| 参考文献 | Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. |
Western blot analysis of extracts from 293 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® RSK1 siRNA I (+) or SignalSilence® RSK1 siRNA II #6313 (+), using RSK1 Antibody #9333 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The RSK1 Antibody confirms silencing of RSK1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.western blot方法检测细胞提取物：转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)和SignalSilence® RSK1 siRNA I (+) 、SignalSilence® RSK1 siRNA II #6313 (+)的293 细胞，使用的抗体是RSK1 Antibody #9333（上图）和α-Tubulin (11H10) Rabbit mAb #2125（下图）。用 RSK1 Antibody证实了pRSK1的沉默表达，而α-Tubulin (11H10) Rabbit mAb被用于负载对照。 |
