| 货号 | 6313S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM RSK1 siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). |
| 存放说明 | -20C |
| 参考文献 | Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. |
Western blot analysis of extracts from 293 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® RSK1 siRNA I #6309 (+) or SignalSilence® RSK1 siRNA II (+), using RSK1 Antibody #9333 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The RSK1 Antibody confirms silencing of RSK1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.western blot方法检测细胞提取物：转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)和SignalSilence® RSK1 siRNA I #6309 (+) 、 SignalSilence® RSK1 siRNA II (+)的293 细胞，使用的抗体是RSK1 Antibody #9333（上图）和α-Tubulin (11H10) Rabbit mAb #2125（下图）。用 RSK1 Antibody证实了pRSK1的沉默表达，而α-Tubulin (11H10) Rabbit mAb被用于负载对照。 |
