SignalSilence® SET8 siRNA I
货号：
13067S 基本售价：
3318.0 元 规格：
300ul
产品信息
概述| 货号 | 13067S |
| 描述 | SignalSilence® SET8 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit SET8 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
性能| 供应商 | CST |
| 存放说明 | -20C |
| 参考文献 | Fang, J. et al. (2002) Curr Biol 12, 1086-99.
Xiao, B. et al. (2005) Genes Dev 19, 1444-54.
Couture, J.F. et al. (2005) Genes Dev 19, 1455-65.
Rice, J.C. et al. (2002) Genes Dev 16, 2225-30.
Huen, M.S. et al. (2008) J Biol Chem 283, 11073-7.
Tardat, M. et al. (2007) J Cell Biol 179, 1413-26.
Jørgensen, S. et al. (2007) J Cell Biol 179, 1337-45.
Shi, X. et al. (2007) Mol Cell 27, 636-46.
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参考图片Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® SET8 siRNA I (+) or SignalSilence®SET8 siRNA II #13107 (+), using SET8 (C18B7) Rabbit mAb #2996 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The SET8 (C18B7) Rabbit mAb, which cross-reacts with an unidentified protein at 50 kDa, confirms silencing of SET8 expression while the β-Actin (D6A8) Rabbit mAb is used as a loading control.Western blot 方法检测293T细胞提取物。用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)，SignalSilence® SignalSilence® SET8 siRNA I (+) 或 SignalSilence® SET8 siRNA II #13107 (+)转染细胞。所用抗体为SET8 (C18B7) Rabbit mAb #2996 (上图) 或β-Actin (D6A8) Rabbit mAb #8457 (下图)。SET8 (C18B7) Rabbit mAb还能够与一个50kDa的未知蛋白结合。SET8 (C18B7) Rabbit mAb确定SET8表达的沉默效率，β-Actin (D6A8) 兔单克隆抗体作为上样量对照。 |
