| 货号 | 12875S |
| 描述 | SignalSilence® Sharpin siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit sharpin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | SHank-Associated RH domain-interacting ProteIN (Sharpin), also known as SIPL1, is a highly conserved gene among many mammalian species and is ubiquitously expressed in various types of cells and tissues. Sharpin harbors multiple functional motifs including an amino terminal coiled-coil (CC) domain, which has been shown to mediate the interaction between sharpin and the scaffold protein shank (1). The other two domains, ubiquitin-like domain (UBL) and NPL4 zinc finger domain (NZF), facilitate ubiquitin-mediated protein recognition and degradation (2). Recent studies have shown that both UBL and NZF domains are essential for sharpin to exert its function in part through ubiquitin-mediated mechanisms (3-5). Although sharpin was initially identified as a scaffold protein within the postsynaptic density of neurons (1), recent studies have identified sharpin as a novel modulator of immune and inflammatory diseases. An emerging mechanistic model suggests that sharpin functions as an important adaptor component of the linear ubiquitin chain assembly complex (LUBAC) that modulates activation of the canonical NF-κB signaling pathway (3,4,6,7), thereby regulating cell survival and apoptosis, cytokine production, and development of lymphoid tissues. Indeed, mice with spontaneous mutations in the Sharpin gene develop chronic proliferative dermatitis that is characterized by eosinophilic inflammation of the skin and dysregulated development of lymphoid tissues (8). |
| 存放说明 | -20C |
| 参考文献 | Lim, S. et al. (2001) Mol Cell Neurosci 17, 385-97. Grabbe, C. and Dikic, I. (2009) Chem Rev 109, 1481-94. Ikeda, F. et al. (2011) Nature 471, 637-41. Tokunaga, F. et al. (2011) Nature 471, 633-6. Iwai, K. (2011) Cell Cycle 10, 3095-104. Gerlach, B. et al. (2011) Nature 471, 591-6. Tokunaga, F. et al. (2009) Nat Cell Biol 11, 123-32. Seymour, R.E. et al. (2007) Genes Immun 8, 416-21. |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Sharpin siRNA I (+), using Sharpin (D4P5B) Rabbit mAb #12541 (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The Sharpin (D4P5B) Rabbit mAb confirms silencing of sharpin expression, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control. Western blot检测转染有100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® Sharpin siRNA I (+)的293T细胞，使用的抗体是Sharpin (D4P5B) Rabbit mAb #12541 (上图)或GAPDH (D16H11) XP® Rabbit mAb #5174 (下图)。Sharpin (D4P5B) Rabbit mAb证实了sharpin的沉默表达，GAPDH (D16H11) XP® Rabbit mAb为上样参照。 |
