| 货号 | 7917S |
| 描述 | SignalSilence® SHP-2 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit SHP-2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.Quality Control |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP). It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens, and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 at p85 binding sites (3). SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy-terminus in response to growth factor receptor activation (4). These phosphorylation events are thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity (5). Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation (6). |
| 存放说明 | -20C |
| 参考文献 | Qu, C.K. (2000) Cell Res 10, 279-88. Maroun, C.R. et al. (2000) Mol Cell Biol 20, 8513-25. Zhang, S.Q. et al. (2002) Mol Cell Biol 22, 4062-72. Bennett, A.M. et al. (1994) Proc Natl Acad Sci USA 91, 7335-9. Lu, W. et al. (2001) Mol Cell 8, 759-69. Edouard, T. et al. (2007) Cell Mol Life Sci 64, 1585-90. |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® SHP-2 siRNA I (+), or SignalSilence® SHP-2 siRNA II #7927 (+), using SHP-2 (D50F2) Rabbit mAb #3397 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The SHP-2 (D50F2) Rabbit mAb confirms silencing of SHP-2 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. |
