| 货号 | 12241S |
| 描述 | SignalSilence® SirT1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit SirT1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® SirT1 siRNA I inhibits human, mouse, rat, and monkey SirT1 expression. |
| 供应商 | CST |
| 背景 | The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7), and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, as it is phosphorylated at Ser27 and Ser47 in vivo; however, the function of these phosphorylation sites has not yet been determined (9). |
| 存放说明 | -20C |
| 参考文献 | Guarente, L. (1999) Nat. Genet. 23, 281-285. Vaziri, H. et al. (2001) Cell 107, 149-159. Luo, J. et al. (2001) Cell 107, 137-148. Bouras, T. et al. (2005) J. Biol. Chem. 280, 10264-10276. Brunet, A. et al. (2004) Science 303, 2011-2015. Motta, M.C. et al. (2004) Cell 116, 551-563. Picard, F. et al. (2004) Nature 429, 771-776. Rodgers, J.T. et al. (2005) Nature 434, 113-118. Beausoleil, S.A. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 12130-12135. |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® SirT1 siRNA I (+), using SirT1 (D1D7) Rabbit mAb #9475 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The SirT1 (D1D7) Rabbit mAb confirms silencing of SirT1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. Western blot 方法检测293T细胞提取物。用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® SirT1 siRNA I (+)转染细胞。所用抗体为SirT1 (D1D7) Rabbit mAb #9475 兔单抗(上图) 或 β-Actin (D6A8) Rabbit mAb #8457兔单抗 (下图)。使用SirT1 (D1D7) Rabbit mAb确定SirT1表达的沉默效率，β-Actin (D6A8) 兔单克隆抗体作为上样量对照。 |
