| 货号 | 9390S |
| 描述 | SignalSilence® SirT6 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit SirT6 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | The Silent Information Regulator (Sir2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of this family is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway (2-4). The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation, and deamination events. SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H2O2 (2). In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres, and gaps (2). SirT6 may regulate the BER pathway by deacetylating DNA Polβ or other core components of the pathway (2). |
| 存放说明 | -20C |
| 参考文献 | Guarente, L. (1999) Nat Genet 23, 281-5. Mostoslavsky, R. et al. (2006) Cell 124, 315-29. Liszt, G. et al. (2005) J Biol Chem 280, 21313-20. Michishita, E. et al. (2005) Mol Biol Cell 16, 4623-35. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® SirT6 siRNA I (+), using SirT6 (D10A4) Rabbit mAb #8771 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The SirT6 (D10A4) Rabbit mAb confirms silencing of SirT6 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. Western blot 方法检测HeLa细胞提取物。用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® SirT6 siRNA I (+)转染细胞。所用抗体为SirT6 (D10A4) Rabbit mAb #8771 兔单抗 (上图) 或 β-Actin (D6A8) Rabbit mAb #8457兔单抗 (下图)。使用SirT6 (D10A4) Rabbit mAb兔单抗确定SirT6表达的沉默效率，β-Actin (D6A8) 兔单克隆抗体作为上样量对照。 |
