SignalSilence® Skp2 siRNA I
货号：
7753S 基本售价：
3318.0 元 规格：
300ul
产品信息
概述| 货号 | 7753S |
| 描述 | SignalSilence® Skp2 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Skp2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
性能| 供应商 | CST |
| 背景 | Members of the F-box family of proteins are characterized by the approximate 40 amino acid F-box motif named after cyclin F (1,2). F-box proteins constitute one of the four subunits of the Skp1-Cullin-F-box (SCF) ubiquitin ligase complex. The substrate specificity of SCF complexes is determined by the interchangeable F-box proteins, which act as adaptors by associating with phosphorylated substrate proteins and recruiting them to the SCF core. F-box proteins contain two fundamental domains: the F-box motif mediates binding to Skp1 and a leucine rich repeat (LRR) domain mediates substrate interactions. |
| 存放说明 | -20C |
| 参考文献 | Pagano, M. (2004) Mol Cell 14, 414-6.
Reed, S.I. (2003) Nat Rev Mol Cell Biol 4, 855-64.
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参考图片Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Skp2 siRNA I (+), or SignalSilence® Skp2 siRNA II #7756 (+), using Skp2 (L70) Antibody #4313 (upper) or GAPDH (14C10) Rabbit mAb #2118 (lower). The Skp2 (L70) Antibody confirms silencing of Skp2 expression, while the GAPDH (14C10) Rabbit mAb is used as a loading control.Western blot检测转染有100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)，SignalSilence® Skp2 siRNA I (+)或SignalSilence® Skp2 siRNA II #7756 (+)的HeLa细胞，采用Skp2 (L70) Antibody #4313 (upper) 或GAPDH (14C10) Rabbit mAb #2118 (lower)。Skp2 (L70) Antibody证实了Skp2沉默表达，采用GAPDH (14C10) Rabbit mAb作为内参照。 |
