SignalSilence® Smad1 siRNA I【科瑞奥博】

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SignalSilence® Smad1 siRNA I

货号： 6223S 基本售价： 3318.0 元规格： 300ul

产品信息

概述
货号	6223S
描述	SignalSilence® Smad1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Smad1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
反应种属	Human
应用	TFN

性能
供应商	CST
背景	Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).
存放说明	-20C
参考文献	Hogan, B.L. (1996) Genes Dev 10, 1580-94. Hoodless, P.A. et al. (1996) Cell 85, 489-500. Klemm, J.D. et al. (1998) Annu Rev Immunol 16, 569-92. Kretzschmar, M. et al. (1997) Genes Dev 11, 984-95. Whitman, M. (1998) Genes Dev 12, 2445-62. Sapkota, G. et al. (2007) Mol Cell 25, 441-54. Alarcón, C. et al. (2009) Cell 139, 757-69.

参考图片

Western blot analysis of extracts from ACHN cells, transfected with 100 nM SignalSilence^® Control siRNA (Unconjugated) #6568 (-), SignalSilence^® Smad1 siRNA I (+), or SignalSilence® Smad1 siRNA II #6227 (+) using Smad1 (D59F7) XP^® Rabbit mAb #6944 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Smad1 (D59F7) XP^® Rabbit mAb confirms silencing of Smad1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

转染100 nM SignalSilence^® Control siRNA (无偶联物) #6568 (-)或 SignalSilence® Smad1 siRNA I (+)或 SignalSilence^® Smad1 siRNA II #6227 (+) 的ACHN细胞，使用Smad1 (D59F7) XP^® Rabbit mAb兔单抗#6944 (上) 或α-Tubulin (11H10) Rabbit mAb兔单抗 #2125 (下)对细胞提取物进行western blot分析。Smad1 (D59F7) XP^® 用以确认Smad1的敲除。α-Tubulin (11H10)Rabbit mAb用作上样对照。