| 货号 | 12104S |
| 描述 | SignalSilence® SP1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit SP1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® SP1 siRNA I inhibits human and monkey SP1 expression. |
| 供应商 | CST |
| 背景 | Specificity protein 1 (SP1) is a ubiquitously expressed transcription factor belonging to the family of C2H2-type zinc finger containing DNA-binding proteins. SP1 binds GC-rich motifs with high affinity and regulates the expression of numerous mammalian genes (1,2). It interacts with many other transcription factors, such as c-Myc, EGR1, and Stat1, and with basal transcription machinery components. SP1 interacts with chromatin-modifying factors, such as histone deacetylases (HDACs) and p300 in chromatin remodeling. Transcriptional activity and stability of SP1 are regulated by post-translational modification, including phosphorylation, acetylation, ubiquitination, and glycosylation (3). Glycosylation of SP1 following insulin treatment leads to increased nuclear localization, while glucagon treatment increases cytoplasmic SP1 levels (4-6). Investigators have found high levels of SP1 in patients with Alzheimers disease (7). |
| 存放说明 | -20C |
| 参考文献 | Kadonaga, J.T. et al. (1987) Cell 51, 1079-90. Song, J. et al. (2003) Int J Mol Med 11, 547-53. Tan, N.Y. and Khachigian, L.M. (2009) Mol Cell Biol 29, 2483-8. Majumdar, G. et al. (2003) Am J Physiol Endocrinol Metab 285, E584-91. Majumdar, G. et al. (2006) J Biol Chem 281, 3642-50. Solomon, S.S. et al. (2008) Life Sci 83, 305-12. Citron, B.A. et al. (2008) J Neurosci Res 86, 2499-504. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® SP1 siRNA I (+), or SignalSilence® SP1 siRNA II #12106 (+), using SP1 Antibody #5931 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The SP1 Antibody confirms silencing of SP1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. The SP1 Antibody cross-reacts with a protein of unknown origin at 55 kDa, which is unaffected by SignalSilence® SP1 siRNA I or SignalSilence® SP1 siRNA II #12106.Western blot 方法检测Hela细胞提取物。用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)，SignalSilence® SP1 siRNA I (+), 或SignalSilence® SP1 siRNA II #12106 (+)转染细胞。所用抗体为SP1 Antibody #5931 (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。SP1抗体确定SP1表达的沉默效率，β-Actin (D6A8) 兔单克隆抗体作为上样量对照。如图所示，SP1抗体还能够与一个55kDa的未知蛋白结合，并且不受SignalSilence® SP1 siRNA I 或 SignalSilence® SP1 siRNA II#12106的影响。 |
