| 货号 | 6394S |
| 描述 | SignalSilence® SQSTM1/p62 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit SQSTM1/p62 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress, and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5), and independently found to interact with PKCζ (6,7). It was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4,10,11). SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity. |
| 存放说明 | -20C |
| 参考文献 | Kirkin, V. et al. (2009) Mol Cell 34, 259-69. Seibenhener, M.L. et al. (2007) FEBS Lett 581, 175-9. Komatsu, M. et al. (2010) Nat Cell Biol 12, 213-23. Bjørkøy, G. et al. (2006) Autophagy 2, 138-9. Joung, I. et al. (1996) Proc Natl Acad Sci USA 93, 5991-5. Sanchez, P. et al. (1998) Mol Cell Biol 18, 3069-80. Puls, A. et al. (1997) Proc Natl Acad Sci USA 94, 6191-6. Vadlamudi, R.K. et al. (1996) J Biol Chem 271, 20235-7. Wooten, M.W. et al. (2005) J Biol Chem 280, 35625-9. Bjørkøy, G. et al. (2005) J Cell Biol 171, 603-14. Komatsu, M. et al. (2007) Cell 131, 1149-63. Pankiv, S. et al. (2007) J Biol Chem 282, 24131-45. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® SQSTM1/p62 siRNA I (+) or SignalSilence® SQSTM1/p62 siRNA II #6399 (+), using SQSTM1/p62 (D5E2) Rabbit mAb #8025 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The SQSTM1/p62 (D5E2) Rabbit mAb confirms silencing of SQSTM1/p62 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot 分析HeLa细胞的细胞提取物，转染100 nM SignalSilence® Control siRNA (未结合) #6568 (-)或 SignalSilence® SQSTM1/p62 siRNA I (+) 或 SignalSilence® SQSTM1/p62 siRNA II #6399 (+)，使用抗体是SQSTM1/p62 (D5E2) Rabbit mAb 兔单抗#8025 (上图)或α-Tubulin (11H10) Rabbit mAb 兔单抗#2125 (下图)。SQSTM1/p62 (D5E2) Rabbit mAb 兔单抗证实能够沉默 SQSTM1/p62 的表达，α-Tubulin (11H10) Rabbit mAb 兔单抗用来检测内参。 |
