SignalSilence® Stat1 siRNA I【科瑞奥博】

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SignalSilence® Stat1 siRNA I

货号： 6331S 基本售价： 3318.0 元规格： 300ul

产品信息

概述
货号	6331S
描述	SignalSilence® Stat1 siRNA allows the researcher to specifically inhibit Stat1 expression by RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce protein expression in specified cell lines.
反应种属	Human
应用	W

性能
供应商	CST
背景	The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
存放说明	-20C
计算分子量	84, 91
参考文献	Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120. Durbin, J.E. et al. (1996) Cell 84, 443-50. Meraz, M.A. et al. (1996) Cell 84, 431-42. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7. Frank, D.A. (1999) Mol Med 5, 432-56. Wen, Z. et al. (1995) Cell 82, 241-50.

参考图片

Western blot analysis of extracts from HeLa cells 48 hours following mock transfection, transfection with non-targeted (control) siRNA or transfection with Stat1 siRNA. Stat1 was detected using Stat1 Antibody #9172, and p42 was detected using p42 MAPK Antibody #9108. The Stat1 Antibody confirms silencing of Stat1 expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity.Western免疫印迹。用 Stat1 Antibody #9172和 p42 MAPK Antibody #9108研究对照转染，转染了 non-targeted (对照) siRNA 或 Stat1 siRNA 48小时后的HeLa 细胞的细胞提取液。 Stat1 Antibody验证 Stat1 的表达, p42 MAPK Antibody 用做上样量的对照和siRNA特异性对照。