| 货号 | 6544S |
| 描述 | SignalSilence® Stat1 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Stat1 expression by RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. |
| 存放说明 | -20C |
| 参考文献 | Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120. Durbin, J.E. et al. (1996) Cell 84, 443-50. Meraz, M.A. et al. (1996) Cell 84, 431-42. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7. Frank, D.A. (1999) Mol Med 5, 432-56. Wen, Z. et al. (1995) Cell 82, 241-50. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Stat1 siRNA I #6331 or SignalSilence® Stat1 siRNA II (+), using Stat1 Antibody #9172 and β-Actin (13E5) Rabbit mAb #4970. The Stat1 Antibody confirms silencing of Stat1 expression and β-Actin (13E5) Rabbit mAb is used to control for loading and specificity of Stat1 siRNA.Western免疫印迹。用Stat1 Antibody #9172 和 β-Actin (13E5) Rabbit mAb #4970 研究转染了100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) 或 SignalSilence® Stat1 siRNA I #6331 或SignalSilence® Stat1 siRNA II (+)的HeLa 细胞的细胞提取液。 |
