| 货号 | 6580S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM Stat3 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. CST推荐使用100 nM SignalSilence® Stat3 siRNA I 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。 |
| 供应商 | CST |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | -20C |
| 参考文献 | Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85. Bromberg, J.F. et al. (1999) Cell 98, 295-303. Darnell, J.E. et al. (1994) Science 264, 1415-21. Ihle, J.N. (1995) Nature 377, 591-4. Wen, Z. et al. (1995) Cell 82, 241-50. Yokogami, K. et al. (2000) Curr Biol 10, 47-50. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Stat3 siRNA I (+) or SignalSilence® Stat3 siRNA II #6582 (+), using Stat3 (79D7) Rabbit mAb #4904 and α-Tubulin (11H10) Rabbit mAb #2125. The Stat3 (79D7) Rabbit mAb confirms silencing of Stat3 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Stat3 siRNA. western blot方法检测细胞提取物： pervanadate(1 mM 5 min)处理，并稳定转染的Src结构性活化形式（酪氨酸527残基突变为phenylalanine）的HeLa细胞，以及A431、HepG2细胞。使用的抗体是Non-phospho-Src (Tyr416) (7G9 ) mAb #2102 (上图), v-Src antibody (中间) 和Phospho-Src (Tyr416) Antibody #2101（下图）。 |
