| 货号 | 6353S |
| 描述 | SignalSilence® Stat3 siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Stat3 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Mouse |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | -20C |
| 参考文献 | Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85. Bromberg, J.F. et al. (1999) Cell 98, 295-303. Darnell, J.E. et al. (1994) Science 264, 1415-21. Ihle, J.N. (1995) Nature 377, 591-4. Wen, Z. et al. (1995) Cell 82, 241-50. Yokogami, K. et al. (2000) Curr Biol 10, 47-50. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94. |
Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Stat3 siRNA I (Mouse Specific) (+), or SignalSilence® Stat3 siRNA II (Mouse Specific) #6354 (+) using Stat3 (124H6) Mouse mAb #9139 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Stat3 (124H6) Mouse mAb confirms silencing of Stat3 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western免疫印迹。用 Stat3 (124H6) Mouse mAb #9139 (上图) 或 α-Tubulin (11H10) Rabbit mAb #2125 (下图) 研究转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Stat3 siRNA I (Mouse Specific) (+)或 SignalSilence® Stat3 siRNA II (Mouse Specific) #6354 (+)的NIH/3T3细胞的细胞提取液。Stat3 (124H6) Mouse mAb 证实对Stat3表达的沉默, α-Tubulin (11H10) Rabbit mAb 作为上样量的对照。 |
