SignalSilence® Stat5 siRNA II【科瑞奥博】

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SignalSilence® Stat5 siRNA II

货号： 6298S 基本售价： 3318.0 元规格： 300ul

产品信息

概述
货号	6298S
描述	SignalSilence® Stat5 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Stat5 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
反应种属	Human
应用	TFN

性能
供应商	CST
背景	Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).
存放说明	-20C
参考文献	Gouilleux, F. et al. (1994) EMBO J 13, 4361-9. Wakao, H. et al. (1994) EMBO J 13, 2182-91. Okutani, Y. et al. (2001) Oncogene 20, 6643-50. Demoulin, J.B. et al. (1999) J Biol Chem 274, 25855-61. Dentelli, P. et al. (1999) J Immunol 163, 2151-9. Meinke, A. et al. (1996) Mol Cell Biol 16, 6937-44.

参考图片

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Stat5 siRNA I #6275 (+), or SignalSilence® Stat5 siRNA II (+), using Stat5 (3H7) Rabbit mAb #9358 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Stat5 (3H7) Rabbit mAb confirms silencing of Stat5 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western免疫印迹。用Stat5 (3H7) Rabbit mAb #9358 (上图) 或 α-Tubulin (11H10) Rabbit mAb #2125 (下图)研究转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Stat5 siRNA I #6275 (+) 或SignalSilence® Stat5 siRNA II (+) 的 HeLa 细胞的细胞提取液。Stat5 (3H7) Rabbit mAb证实了对 Stat5 表达的沉默, α-Tubulin (11H10) Rabbit mAb 作为上样量的对照。