| 货号 | 9404S |
| 描述 | SignalSilence® Stat6 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Stat6 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human/Mouse |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® Stat6 siRNA II inhibits human, mouse, rat, and monkey Stat6 expression. |
| 供应商 | CST |
| 背景 | Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cells, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7). |
| 存放说明 | -20C |
| 参考文献 | Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872. Hou, J. et al. (1994) Science 265, 1701-1706. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343. Takeda, K. et al. (1996) Nature 380, 627-630. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791. |
Western blot analysis of extracts from L-929 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Stat6 siRNA I (Mouse Specific) #9396 (+), or SignalSilence® Stat6 siRNA II (+), using Stat6 (D3H4) Rabbit mAb #5397 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The Stat6 (D3H4) Rabbit mAb confirms silencing of Stat6 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.Western blot分析L-929的细胞提取物，转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Stat6 siRNA I (Mouse Specific) (+)#9396, 或 SignalSilence® Stat6 siRNA II (+)，使用抗体是Stat6 (D3H4) Rabbit mAb #5397 (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。Stat6 (D3H4) Rabbit mAb证实了Stat6表达的沉默，β-Actin (D6A8) Rabbit mAb用来检测内参。 |
