| 货号 | 6351S |
| 描述 | SignalSilence® Survivin siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit survivin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 16 |
| 参考文献 | Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200. Li, F. et al. (1998) Nature 396, 580-584. Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466. OConnor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107. Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809. Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640. |
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with nonspecific (control) siRNA or SignalSilence® Survivin siRNA I, using Survivin (6E4) Mouse mAb #2802 and p42 MAP Kinase (Erk2) Antibody #9108. The survivin antibody confirms silencing of survivin expression, while the p42 MAPK antibody is used to control for loading and specificity of survivin siRNA. Western blot方法检测HeLa细胞提取物，空质粒、非特异性（对照）siRNA 或Survivin siRNA转染，转染后48h提取；使用Survivin (6E4) Monoclonal Antibody #2802检测Survivin表达，或用p42 MAPK Antibody #9108检测p42表达。Survivin Antibody确定Survivin表达的沉默，p42 MAPK作为内参对照。 | |
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. 荧光检测SignalSilence® Control siRNA (Fluorescein Conjugate) #6201转染24小时后的活HeLa细胞，显示了近 100%的转染效率。 |
