| 货号 | 6546S |
| 描述 | SignalSilence® Survivin siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit survivin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | Survivin siRNA II will inhibit human and dog Survivin expression. |
| 供应商 | CST |
| 背景 | Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6). |
| 存放说明 | -20C |
| 参考文献 | Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200. Li, F. et al. (1998) Nature 396, 580-584. Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466. OConnor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107. Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809. Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Survivin siRNA II (+), using Survivin (71G4B7E) Rabbit mAb #2808 and α-Tubulin (11H10) Rabbit mAb #2125. Survivin (71G4B7E) Rabbit mAb confirms silencing of survivin expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of survivin siRNA. Western blot方法检测HeLa细胞提取物，100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-)或SignalSilence® Survivin siRNA II (+)转染；使用Survivin (71G4B7E) Rabbit mAb 兔单抗#2808或α-Tubulin (11H10) Rabbit mAb 兔单抗#2125。Survivin (71G4B7E) Rabbit mAb兔单抗确定Survivin表达的沉默，α-Tubulin (11H10) Rabbit mAb 兔单抗作为内参对照。 |
