| 货号 | 6317S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM TAK1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2). |
| 存放说明 | -20C |
| 参考文献 | Yamaguchi, K. et al. (1995) Science 270, 2008-2011. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256. Shibuya, H. et al. (1996) Science 272, 1179-1182. Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145. Takaesu, G. et al. (2000) Mol. Cell 4, 649-658. |
Western blot analysis of extracts from 293 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® TAK1 siRNA I (+) or SignalSilence® TAK1 siRNA II #6318 (+), using TAK1 Antibody #4505 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The TAK1 Antibody confirms silencing of TAK1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.western blot方法检测细胞提取物： 转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® TAK1 siRNA I (+) 、SignalSilence® TAK1 siRNA II #6318 (+)的293细胞。使用的抗体是TAK1 Antibody #4505 (上图) 和α-Tubulin (11H10) Rabbit mAb #2125（下图）。TAK1 Antibody证实了TAK1的表达，α-Tubulin (11H10) Rabbit mAb用于负载对照。 |
