SignalSilence® TAK1 siRNA II【科瑞奥博】

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SignalSilence® TAK1 siRNA II

货号： 6318S 基本售价： 3318.0 元规格： 300ul

产品信息

概述
货号	6318S
反应种属	Human
应用	TFN
使用方法	CST recommends transfection with 100 nM TAK1 siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. CST推荐使用100 nM SignalSilence^® TAK1 siRNA II 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。

性能
供应商	CST
背景	TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2).
存放说明	-20C
参考文献	Yamaguchi, K. et al. (1995) Science 270, 2008-2011. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256. Shibuya, H. et al. (1996) Science 272, 1179-1182. Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145. Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.

参考图片

Western blot analysis of extracts from 293 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® TAK1 siRNA I #6317 (+) or SignalSilence® TAK1 siRNA II (+), using TAK1 Antibody #4505 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The TAK1 Antibody confirms silencing of TAK1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.western blot方法检测细胞提取物：转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® TAK1 siRNA I #6317 (+) 和SignalSilence® TAK1 siRNA II (+)的293细胞。使用的抗体是TAK1 Antibody #4505 (上图) 和α-Tubulin (11H10) Rabbit mAb #2125（下图）。TAK1 Antibody证实了TAK1的表达，α-Tubulin (11H10) Rabbit mAb用于负载对照。