| 货号 | 6236S |
| 描述 | SignalSilence® Toll-like Receptor 3 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Toll-like Receptor 3 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB that normally maintains NF-κB inactivity by sequestering it in the cytoplasm. |
| 存放说明 | -20C |
| 参考文献 | Akira, S. (2003) J Biol Chem 278, 38105-8. Beutler, B. (2004) Nature 430, 257-63. Dunne, A. and ONeill, L.A. (2003) Sci STKE 2003, re3. Medzhitov, R. et al. (1997) Nature 388, 394-7. Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9. Takeuchi, O. et al. (1999) Immunity 11, 443-51. Alexopoulou, L. et al. (2001) Nature 413, 732-8. Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4. Horng, T. et al. (2001) Nat Immunol 2, 835-41. Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7. Muzio, M. et al. (1997) Science 278, 1612-5. Wesche, H. et al. (1997) Immunity 7, 837-47. Suzuki, N. et al. (2002) Nature 416, 750-6. Irie, T. et al. (2000) FEBS Lett 467, 160-4. |
Western blot analysis of extracts from MCF 10A cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Toll-like Receptor 3 siRNA I (+), using Toll-like Receptor 3 (D10F10) Rabbit mAb #6961 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Toll-like Receptor 3 (D10F10) Rabbit mAb confirms silencing ofToll-like Receptor 3 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western免疫印迹。用Toll-like Receptor 3 (D10F10) Rabbit mAb #6961 (上图)或α-Tubulin (11H10) Rabbit mAb #2125 (下图)研究转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或 SignalSilence® Toll-like Receptor 3 siRNA I (+)的MCF 10A细胞的细胞提取液。 Toll-like Receptor 3 (D10F10) Rabbit mAb证实了对Toll-like Receptor 3表达的沉默,α-Tubulin (11H10) Rabbit mAb作为上样量的对照。 |
