| 货号 | 6476S |
| 描述 | SignalSilence® Tuberin/TSC2 siRNA from Cell Signaling Technology (CST) allows the researcher to specifically inhibit tuberin/TSC2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).Small interfering RNA (siRNA) has been used to specifically silence tuberin in HEK293 cells (7). |
| 存放说明 | -20C |
| 计算分子量 | 200 |
| 参考文献 | Soucek, T. et al. (1998) Proc Natl Acad Sci U S A 95, 15653-8. Sparagana, S.P. and Roach, E.S. (2000) Curr Opin Neurol 13, 115-9. Manning, B.D. et al. (2002) Mol Cell 10, 151-62. Aicher, L.D. et al. (2001) J Biol Chem 276, 21017-21. Dan, H.C. et al. (2002) J Biol Chem 277, 35364-70. Goncharova, E.A. et al. (2002) J Biol Chem 277, 30958-67. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57. |
Western blot analysis of extracts from HeLa cells, transfected with non-targeted (-) or SignalSilence® Tuberin/TSC2 siRNA I (+) using a tuberin/TSC2 antibody and α-Tubulin (11H10) Rabbit mAb #2125. The tuberin/TSC2 antibody confirms silencing of tuberin/TSC2 expression, while the α-Tubulin antibody is used as a loading control. Western blot 分析HeLa细胞提取物，转染采用non-targeted (-) or SignalSilence® Tuberin/TSC2 siRNA I (+)，所用抗体为a tuberin/TSC2 antibody和 α-Tubulin (11H10) Rabbit mAb #2125。Tuberin/TSC2 antibody 验证了Tuberin/TSC2表达的沉默，α-Tubulin (11H10) Rabbit mAb 兔单抗作为上样对照。 |
