| 货号 | 6548S |
| 描述 | SignalSilence® Tuberin/TSC2 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit tuberin/TSC2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7). |
| 存放说明 | -20C |
| 参考文献 | Soucek, T. et al. (1998) Proc Natl Acad Sci U S A 95, 15653-8. Sparagana, S.P. and Roach, E.S. (2000) Curr Opin Neurol 13, 115-9. Manning, B.D. et al. (2002) Mol Cell 10, 151-62. Aicher, L.D. et al. (2001) J Biol Chem 276, 21017-21. Dan, H.C. et al. (2002) J Biol Chem 277, 35364-70. Goncharova, E.A. et al. (2002) J Biol Chem 277, 30958-67. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Tuberin/TSC2 siRNA I #6476 or SignalSilence® Tuberin/TSC2 siRNA II (+), using Tuberin/TSC2 (28A7) Rabbit mAb #3635 and α-Tubulin (11H10) Rabbit mAb #2125. Tuberin/TSC2 (28A7) Rabbit mAb confirms silencing of Tuberin/TSC2 expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Tuberin/TSC2 siRNA. Western blot 分析HeLa细胞提取物，转染采用100 nM SignalSilence ® Control siRNA (Fluorescein Con-jugate) #6201 (-) 或 SignalSilence ® Tuberin/TSC2 siRNA I #6476或SignalSilence ® Tuberin/TSC2 siRNA II (+)所用抗体为Tuberin/TSC2 (28A7) Rabbit mAb #3635和 α-Tubulin (11H10) Rabbit mAb #2125。 Tuberin/TSC2 (28A7) Rabbit mAb验证了Tuberin/TSC2表达的沉默， α-Tubulin (11H10) Rabbit mAb作为上样对照和 mTOR siRNA的特异性。 |
