| 货号 | 14254S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM SignalSilence® Tyk2 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow the protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | Tyk2 is a member of the Jak family of protein tyrosine kinases. It associates with and is activated by receptors for many cytokines including IL-13, the IL-6 family, IL-10, and IFN-α and β (1-3). Following ligand binding, Tyk2 is activated by phosphorylation of Tyr1054 and/or Tyr1055 (4). Tyk2 is required for the tyrosine phosphorylation of Stat3 in the IFN-β signaling cascade (5). |
| 存放说明 | -20C |
| 参考文献 | Velazquez, L. et al. (1995) J. Biol. Chem. 270, 3327-34. Stahl, N. et al. (1994) Science 263, 92-5. Leonard, W.J. (1998) Annu. Rev. Immunol. 16, 293-322. Grauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-500. Rani, M.R. et al. (1999) J. Biol. Chem. 274, 32507-11. Strobl, B. et al. (2011) Front Biosci (Landmark Ed) 16, 3214-32. Karaghiosoff, M. et al. (2000) Immunity 13, 549-60. Shimoda, K. et al. (2000) Immunity 13, 561-71. Minegishi, Y. et al. (2006) Immunity 25, 745-55. |
Western blot analysis of extracts from MCF7 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), or SignalSilence® Tyk2 siRNA I (+), using Tyk2 (D4I5T) Rabbit mAb #14193 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The Tyk2 (D4I5T) Rabbit mAb confirms silencing of Tyk2 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. |
