| 货号 | 7000S |
| 描述 | SignalSilence® ULK1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit ULK1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® ULK1 siRNA I inhibits human and monkey ULK1 expression. |
| 供应商 | CST |
| 背景 | Two related serine/threonine kinases, UNC-51-like kinase 1 and 2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene UNC-51 in which mutants exhibited abnormal axonal extension and growth (1-4). Both proteins are widely expressed and contain an amino-terminal kinase domain followed by a central proline/serine rich domain and a highly conserved carboxy-terminal domain. The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors, such as NGF (5). Yeast two-hybrid studies found ULK1/2 associated with modulators of the endocytic pathway, SynGap and syntenin (6). Structural similarity of ULK1/2 has also been recognized with the yeast autophagy protein Atg1/Apg1 (7). Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy (8), a catabolic process for the degradation of bulk cytoplasmic contents (9,10). It appears that Atg1/ULK1 can act as a convergence point for multiple signals that control autophagy (11), and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking (12-16). |
| 存放说明 | -20C |
| 参考文献 | Ogura, K. et al. (1994) Genes Dev 8, 2389-400. Kuroyanagi, H. et al. (1998) Genomics 51, 76-85. Yan, J. et al. (1998) Biochem Biophys Res Commun 246, 222-7. Yan, J. et al. (1999) Oncogene 18, 5850-9. Zhou, X. et al. (2007) Proc Natl Acad Sci USA 104, 5842-7. Tomoda, T. et al. (2004) Genes Dev 18, 541-58. Matsuura, A. et al. (1997) Gene 192, 245-50. Chan, E.Y. et al. (2007) J Biol Chem 282, 25464-74. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. Stephan, J.S. and Herman, P.K. (2006) Autophagy 2, 146-8. Okazaki, N. et al. (2000) Brain Res Mol Brain Res 85, 1-12. Young, A.R. et al. (2006) J Cell Sci 119, 3888-900. Kamada, Y. et al. (2000) J Cell Biol 150, 1507-13. Lee, S.B. et al. (2007) EMBO Rep 8, 360-5. Hara, T. et al. (2008) J Cell Biol 181, 497-510. Kim, J. et al. (2011) Nat Cell Biol 13, 132-41. Egan, D.F. et al. (2011) Science 331, 456-61. |
Western blot analysis of extracts from RD cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® ULK1 siRNA I (+), using ULK1 (D8H5) Rabbit mAb #8054 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The ULK1 (D8H5) Rabbit mAb confirms silencing of ULK1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. Western blot分析RD细胞的细胞提取物，转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® ULK1 siRNA I (+)，使用抗体是ULK1 (D8H5) Rabbit mAb 兔单抗#8054 (上图) 或 β-Actin (D6A8) Rabbit mAb 兔单抗#8457 (下图)。ULK1 (D8H5) Rabbit mAb 兔单抗确认ULK1表达的沉默，β-Actin (D6A8) Rabbit mAb 兔单抗用来检测内参。 | |
Western blot analysis of extracts from RD cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® ULK1 siRNA I (+), using ULK1 (D9D7) Rabbit mAb #6439 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The ULK1 (D9D7) Rabbit mAb confirms silencing of ULK1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. Western blot 分析RD细胞的细胞提取物，转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或 SignalSilence® ULK1 siRNA I (+)，使用抗体ULK1 (D9D7) Rabbit mAb 兔单抗#6439 (上图) 或 β-Actin (D6A8) Rabbit mAb 兔单抗#8457 (下图)。ULK1(D9D7) Rabbit mAb 兔单抗确认ULK1表达的沉默，β-Actin (D6A8) Rabbit mAb 兔单抗用来检测内参。 |
