| 货号 | 6490S |
| 描述 | SignalSilence® USP1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit USP1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® USP1 siRNA I inhibits human and monkey USP1 expression. |
| 供应商 | CST |
| 背景 | Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action (1,2). There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP1 belongs to the USP subfamily and is regulated in a cell cycle dependent manner by both transcriptional and ubiquitin-proteosomal mechanisms (3). USP1 is a nuclear protein and localizes to chromatin where it is specifically associated with FANCD2. USP1 deubiquitinates monoubiquitinated FANCD2, which plays an important role in DNA damage repair and Chk1 protein stability (3,4). Another important target of USP1 is PCNA. USP1 deubiquitinates monoubiquitinated PCNA, thereby negatively regulating PCNA-mediated translesion synthesis (TLS) during DNA repair (5). USP1 interaction with UAF1, a WD40 repeat-containing protein, leads to formation of an activated USP1/UAF1 complex, which is required for the deubiquitinase activity of USP1 (6,7). |
| 存放说明 | -20C |
| 参考文献 | Nijman, S.M. et al. (2005) Cell 123, 773-86. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613. Nijman, S.M. et al. (2005) Mol Cell 17, 331-9. Guervilly, J.H. et al. (2011) Hum Mol Genet , . Huang, T.T. et al. (2006) Nat Cell Biol 8, 339-47. Cohn, M.A. et al. (2007) Mol Cell 28, 786-97. Cohn, M.A. et al. (2009) J Biol Chem 284, 5343-51. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® USP1 siRNA I (+) or SignalSilence® USP1 siRNA II #6498 (+), using USP1 (D37B4) Rabbit mAb #8033 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The USP1 (D37B4) Rabbit mAb confirms silencing of USP1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.Western blot检测转染有100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)，SignalSilence® USP1 siRNA I (+)或SignalSilence® USP1 siRNA II #6498 (+)的HeLa细胞，采用USP1 (D37B4) Rabbit mAb #8033 (upper)或β-Actin (D6A8) Rabbit mAb #8457 (lower)。USP1 (D37B4) Rabbit mAb证实了USP1的沉默表达，采用β-Actin (D6A8) Rabbit mAb作为内参照。 |
