| 货号 | 7158S |
| 描述 | SignalSilence® USP14 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit USP14 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. In humans, there are three proteasomal DUBs: PSMD14 (POH1/RPN11), UCH37 (UCH-L5), and Ubiquitin-Specific Protease 14, which is also known as the 60 kDa subunit of tRNA-guanine transglycosylase (USP14/TGT60 kDa). USP14 is recruited to the proteasome through its reversible association with the PSMD2 (S2/hRPN1) subunit of the 19S regulatory particle. Whereas PSMD14 appears to promote substrate degradation (3,4), USP14 is thought to antagonize substrate degradation (5-8). While the underlying mechanism for the opposing roles of these two proteasomal DUBs is still uncertain, it is thought that USP14 removes ubiquitin from substrate upon docking of the substrate with the 26S proteasome. Furthermore, USP14 trims ubiquitin residues from the distal end of the polyubiquitin chain, thus decreasing the affinity of the chain for the ubiquitin receptors of the proteasome, and allowing for enhanced substrate stability (6,9,10). Studies have elucidated a physiologic role for USP14 in regulating synaptic activity in mammals (11); targeting this activity with small molecule inhibitors has potential benefits for the treatment of neurodegenerative diseases and cancer (5,12). |
| 存放说明 | -20C |
| 参考文献 | Nijman, S.M. et al. (2005) Cell 123, 773-86. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613. Verma, R. et al. (2002) Science 298, 611-5. Yao, T. and Cohen, R.E. (2002) Nature 419, 403-7. Lee, B.H. et al. (2010) Nature 467, 179-84. Lam, Y.A. et al. (1997) Nature 385, 737-40. Koulich, E. et al. (2008) Mol Biol Cell 19, 1072-82. Jacobson, A.D. et al. (2009) J Biol Chem 284, 35485-94. Hanna, J. et al. (2006) Cell 127, 99-111. Thrower, J.S. et al. (2000) EMBO J 19, 94-102. Wilson, S.M. et al. (2002) Nat Genet 32, 420-5. DArcy, P. et al. (2011) Nat Med, Epub ahead of print. |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® USP14 siRNA I (+), using USP14 Antibody #8159 and GAPDH (14C10) Rabbit mAb #2118. TheUSP14 Antibody confirms silencing of USP14 expression, while the GAPDH (14C10) Rabbit mAb is used as a loading control.Western blot检测转染有100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® USP14 siRNA I (+), 采用USP14 Antibody #8159和GAPDH (14C10) Rabbit mAb #2118。USP14 Antibody证实了USP14的沉默表达，采用GAPDH (14C10) Rabbit mAb作为内参照。 |
