| 货号 | 7658S |
| 描述 | SignalSilence® VASP siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit VASP expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® VASP siRNA II inhibits human, mouse, rat, and monkey VASP expression. |
| 供应商 | CST |
| 背景 | Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7). |
| 存放说明 | -20C |
| 参考文献 | Butt, E. et al. (1994) J Biol Chem 269, 14509-17. Ball, L.J. et al. (2000) EMBO J 19, 4903-14. Machesky, L.M. (2000) Cell 101, 685-8. Smolenski, A. et al. (1998) J Biol Chem 273, 20029-35. Harbeck, B. et al. (2000) J Biol Chem 275, 30817-25. Oelze, M. et al. (2000) Circ Res 87, 999-1005. Lawrence, D.W. and Pryzwansky, K.B. (2001) J Immunol 166, 5550-6. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® VASP siRNA I #7637 (+) or SignalSilence® VASP siRNA II (+), using VASP (9A2) Rabbit mAb #3132 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The VASP (9A2) Rabbit mAb confirms silencing of VASP expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. 使用VASP (9A2) Rabbit mAb#3132 (上图)或α-Tubulin (11H10) Rabbit mAb #2125 (下图)，免疫印迹（Western blot）分析HeLa细胞中VASP蛋白水平，细胞分别转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)、SignalSilence® VASP siRNA I #7637 (+)或SignalSilence® VASP siRNA II (+)。VASP (9A2) Rabbit mAb确定VASP基因沉默，而α-Tubulin (11H10) Rabbit mAb通常用于内参照。 |
