| 货号 | 14010S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM SignalSilence® VPRBP siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | The HIV-1 viral protein R (Vpr)-binding protein (VPRBP, DCAF1) is a substrate-specific adaptor for the CUL4-based ubiquitin ligase complex that consists of CUL4A, RBX1, and DDB1 (1). VPRBP protein structure contains a central LIS1 homology (LisH) motif responsible for dimerization, and two carboxy-terminal WD-40 motifs involved in Vpr and DDB1 binding (2-4). Research studies demonstrate that VPRBP plays a role in hepatic lipid metabolism by promoting the ubiquitin-dependent proteasomal degradation of the TR4 nuclear receptor, which is involved in lipid homeostasis (5). The VPRBP protein plays a role in mammalian germ cell development through regulation of TET methylcytosine dioxygenase activation (6). Additional studies show that VPRBP exhibits kinase activity and phosphorylates histone H2A at Ser120, which blocks tumor suppressor gene transcription (7). The tumor suppressor Merlin/NF2 inhibits tumorigenesis through interaction with and suppression of the CUL4A-RBX1-DDB1-VPRBP complex (8). |
| 存放说明 | -20C |
| 参考文献 | Lee, J. and Zhou, P. (2007) Mol Cell 26, 775-80. Le Rouzic, E. et al. (2007) Cell Cycle 6, 182-8. Zhang, S. et al. (2001) Gene 263, 131-40. Le Rouzic, E. et al. (2008) J Biol Chem 283, 21686-92. Yoshizawa, T. et al. (2014) Cell Metab 19, 712-21. Yu, C. et al. (2013) Science 342, 1518-21. Kim, K. et al. (2013) Mol Cell 52, 459-67. Li, W. et al. (2010) Cell 140, 477-90. |
Western blot analysis of extracts from HL-60 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® VPRBP siRNA I (+), using VPRBP Antibody #13931 and GAPDH (D16H11) XP® Rabbit mAb #5174. The VPRBP Antibody confirms silencing of VPRBP expression, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control. |
