| 货号 | 6446S |
| 描述 | SignalSilence® XIAP siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit XIAP expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2). |
| 存放说明 | -20C |
| 计算分子量 | 53 |
| 参考文献 | Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46. Deveraux, Q.L. et al. (1997) Nature 388, 300-4. Burstein, E. et al. (2004) EMBO J 23, 244-54. |
Western blot analysis of extracts from A549 cells, mock transfected or transfected with non-targeted (control) siRNA or SignalSilence® XIAP siRNA I, using XIAP Antibody #2042 and Cofilin Antibody #3312. The XIAP antibody confirms silencing of XIAP expression, while the cofilin antibody is used to control for protein loading and specificity of XIAP siRNA. Western blot方法检测A549细胞提取物，用空质粒、非靶向 (对照) siRNA或 SignalSilence® XIAP siRNA I转染，48hr后检测；使用XIAP Antibody #2042和Cofilin Antibody #3312 。XIAP Antibody确定XIAP表达的沉默，cofilin Antibody作为 XIAP siRNA特异性的内参对照。 | |
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. 荧光检测转染24hr后的活HeLa细胞，使用了 SignalSilence® Control siRNA (Fluorescein Conjugate) #6201，结果显示了近100%的转染效率。 |
