| 货号 | 6550S |
| 描述 | SignalSilence® XIAP siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit XIAP expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2). |
| 存放说明 | -20C |
| 参考文献 | Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46. Deveraux, Q.L. et al. (1997) Nature 388, 300-4. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® XIAP siRNA II (+), using XIAP Antibody #2042 and β-Actin Antibody #4967. The XIAP antibody confirms silencing of XIAP expression, while the β-Actin Antibody is used to control for loading and specificity of XIAP siRNA. Western blot方法检测HeLa细胞提取物，100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-)或SignalSilence® XIAP siRNA II (+)转染；使用XIAP Antibody #2042或β-Actin Antibody #4967。XIAP antibody确定XIAP表达的沉默，β-Actin Antibody作为XIAP siRNA的内参对照。 |
