| 货号 | 6206S |
| 描述 | SignalSilence® YB1 siRNA from Cell Signaling Technology (CST) allows the researcher to specifically inhibit YB1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce target protein expression in specified cell lines. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® YB1 siRNA I will inhibit human, mouse and rat YB1 expression. |
| 供应商 | CST |
| 背景 | The Y-box binding protein 1 (YB1) belongs to a family of evolutionarily conserved, multifunctional Y-box proteins that bind single-stranded DNA and RNA and function as regulators of transcription, RNA metabolism, and protein synthesis (1). YB1 binds to Y-box sequences (TAACC) found in multiple gene promoters and can positively or negatively regulate transcription. YB1 activates genes associated with proliferation and cancer, such as cyclin A, cyclin B1, matrix metalloproteinase-2 (MMP-2), and the multi-drug resistance 1 (MDR1) gene (2-4). YB1 represses genes associated with cell death, including the Fas cell death-associated receptor and the p53 tumor suppressor gene (5-7). It also interacts with the RNA-splicing factor SRp30c and stabilizes interleukin 2 mRNA upon induction of T lymphocytes by interleukin 2 (8,9). The majority of YB1 protein localizes to the cytoplasm, with a minor pool found in the nucleus; however, nuclear localization appears to be critical for its role in promoting proliferation. Nuclear translocation is cell cycle regulated, with YB1 protein accumulating in the nucleus during G1/S phase (2). In addition, nuclear translocation is induced in response to extracellular stimuli such as hyperthermia and UV irradiation, or treatment of cells with thrombin, interferons, or insulin-like growth factor (IGF-1) (2,10). Treatment of the MCF7 breast cancer cell line with IGF-1 results in Akt-mediated phosphorylation of YB1 on Ser102, which is required for nuclear translocation of YB1 and its ability to promote anchorage-independent growth (10). YB1 is overexpressed in many malignant tissues, including breast cancer, non-small cell lung carcinoma, ovarian adenocarcinomas, human osteosarcomas, colorectal carcinomas, and malignant melanomas. Nuclear YB1 expression correlates with high levels of proliferation, drug resistance, and poor tumor prognosis (2,7,10). |
| 存放说明 | -20C |
| 参考文献 | Matsumoto, K. and Wolffe, A.P. (1998) Trends Cell Biol. 8, 318-23. Jurchott, K. et al. (2003) J. Biol. Chem. 278, 27988-96. Mertens, P.R. et al. (1997) J. Biol. Chem. 272, 22905-12. Uchiumi, T. et al. (1993) Cell Growth Differ. 4, 147-57. Lasham, A. et al. (2000) Gene 252, 1-13. Lasham, A. et al. (2003) J. Biol. Chem. 278, 35516-23. Homer, C. et al. (2005) Oncogene 24, 8314-25. Raffetseder, U. et al. (2003) J. Biol. Chem. 278, 18241-8. Chen, C.Y. et al. (2000) Genes Dev. 14, 1236-48. Sutherland, B.W. et al. (2005) Oncogene 24, 4281-92. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® YB1 siRNA I (+) or SignalSilence® YB1 siRNA II #6207 (+), using YB1 Antibody #2749 and GAPDH (14C10) Rabbit mAb #2118. The YB1 antibody confirms silencing of YB1 expression and GAPDH (14C10) rabbit mAb is used to control for loading and specificity of YB1 siRNA. Western blot 分析采用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)、SignalSilence® YB1 siRNA I (+) 或SignalSilence® YB1 siRNA II #6207 (+)转染后的Hela细胞提取物，所用抗体为YB1 Antibody #2749 和 GAPDH (14C10) Rabbit mAb 兔单抗#2118。YB1抗体确定了YB1表达的沉默，GAPDH (14C10) rabbit mAb 兔单抗作为YB1 siRNA的对照。 |
