| 货号 | 6218S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM β-Arrestin 1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. CST推荐使用100 nM SignalSilence® β-Arrestin 1 siRNA I 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。 |
| 供应商 | CST |
| 背景 | Arrestin proteins function as negative regulators of G protein coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1). Four distinct mammalian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (or X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as β-arrestin 1) and arrestin 3 (or β-arrestin 2) are ubiquitously expressed and bind to most GPCRs (2). β-arrestin proteins function as adapters and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in ERK activation pathways (3,4). β-arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that β-arrestin proteins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10). |
| 存放说明 | -20C |
| 参考文献 | Shenoy, S.K. and Lefkowitz, R.J. (2005) Sci STKE 2005, cm10. Lefkowitz, R.J. and Shenoy, S.K. (2005) Science 308, 512-7. Luttrell, L.M. et al. (1999) Science 283, 655-61. Luttrell, L.M. et al. (1999) Curr Opin Cell Biol 11, 177-83. Luttrell, L.M. and Lefkowitz, R.J. (2002) J Cell Sci 115, 455-65. Waters, C. et al. (2004) Semin Cell Dev Biol 15, 309-23. Lefkowitz, R.J. and Whalen, E.J. (2004) Curr Opin Cell Biol 16, 162-8. Waters, C.M. et al. (2005) Cell Signal 17, 263-77. Kang, J. et al. (2005) Cell 123, 833-47. Ma, L. and Pei, G. (2007) J Cell Sci 120, 213-8. |
Western blot analysis of extracts from HeLa cells transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® β-Arrestin 1 siRNA I (+) using β-Arrestin 1/2 (D24H9) XP® Rabbit mAb #4674 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The β-Arrestin 1/2 (D24H9) Rabbit mAb confirms silencing of β-Arrestin expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of β-Arrestin 1 siRNA. western blot方法检测细胞提取物：转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)和SignalSilence® β-Arrestin 1 siRNA I (+) 的HeLa细胞，使用的抗体是β-Arrestin 1/2 (D24H9) XP® Rabbit mAb #4674 (上图) or α-Tubulin (11H10) Rabbit mAb #2125 (下图). 已证实β-Arrestin 1/2 (D24H9) Rabbit mAb能使β-Arrestin表达沉默，而α-Tubulin (11H10) Rabbit mAb 被用于负载以及β-Arrestin 1 siRNA I的特异性控制。 | |
Western blot analysis of extracts from HeLa cells transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® β-Arrestin 1 siRNA I (+) using β-Arrestin 2 (C16D9) Rabbit mAb #3857 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The β-Arrestin 2 (C16D9) Rabbit mAb confirms specificity of β-Arrestin 1 siRNA I, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. western blot方法检测细胞提取物：转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)和SignalSilence® β-Arrestin 1 siRNA I (+) 的HeLa细胞，使用的抗体是β-Arrestin 2 (C16D9) Rabbit mAb #3857 (上图) 和α-Tubulin (11H10) Rabbit mAb #2125 (下图). 已证实β-Arrestin 2 (C16D9) Rabbit mAb是β-Arrestin 1 siRNA I的特异性抗体，而α-Tubulin (11H10) Rabbit mAb被用于负载控制。 | |
Western blot analysis of extracts from HeLa cells transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® β-Arrestin 1 siRNA I (+) using β-Arrestin 2 (C16D9) Rabbit mAb #3857 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The β-Arrestin 2 (C16D9) Rabbit mAb confirms specificity of β-Arrestin 1 siRNA I, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. |
