| 货号 | 6225S |
| 描述 | SignalSilence® β-Catenin siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit β-catenin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). |
| 存放说明 | -20C |
| 参考文献 | Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. Amit, S. et al. (2002) Genes Dev 16, 1066-76. Liu, C. et al. (2002) Cell 108, 837-47. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42. Yost, C. et al. (1996) Genes Dev 10, 1443-54. Morin, P.J. et al. (1997) Science 275, 1787-90. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® β-Catenin siRNA I (+) or SignalSilence® β-Catenin siRNA II #6238 (+), using β-Catenin (6B3) Rabbit mAb #9582 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The β-catenin antibody confirms silencing of β-catenin expression, while the α-tubulin rabbit mAb is used is used as a loading control. 转染100 nM SignalSilence® Control siRNA (无偶联物) #6568 (-)或 SignalSilence® β-Catenin siRNA I (+)或SignalSilence® β-Catenin siRNA II #6238 (+)的HeLa细胞，获得提取物使用β-Catenin (6B3) Rabbit mAb#9582 (上) 或 α-Tubulin (11H10) Rabbit mAb#2125 （下）进行western blot实验。β-catenin 抗体用以确认β-catenin 的敲除，α-tubulin 用作上样参照。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® β-Catenin siRNA I (+) or SignalSilence® β-Catenin siRNA II #6238 (+), using β-Catenin Antibody #9562 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The β-catenin antibody recognizes both β-catenin and γ-catenin and demonstrates the specificity of β-catenin expression silencing. The α-tubulin rabbit mAb is used is used as a loading control. 转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或 SignalSilence® β-Catenin siRNA I (+) 或SignalSilence® β-Catenin siRNA II #6238 (+)的HeLa细胞，获得提取物使用β-Catenin抗体#9562 (上)或 α-Tubulin (11H10) 兔单抗#2125 (下)进行western blot实验。β-catenin抗体可以检测β-catenin和 γ-catenin ，用以说明 β-catenin 的特异性敲除。α-tubulin用作上样对照。 |
