| 货号 | 12523S |
| 描述 | SignalSilence® γ-Catenin siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit γ-catenin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Mouse |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Also known as plakoglobin, γ-catenin is a member of the Armadillo family of signaling molecules, which includes β-catenin and the Drosophila protein armadillo (1). This family of proteins is involved in Wnt signaling, which is important in embryonic development and in tumorigenesis (2-3). Although the two vertebrate proteins β- and γ-catenin display sequence homology, γ-catenin likely plays a role distinct from that of β-catenin (1, 4-6). γ-catenin localizes to desmosomes and adherens junctions, both sites of intercellular adhesion, and interacts with the cytoplasmic domains of classical and desmosomal cadherins. Interaction of γ- or β-catenin with α-catenin, desmoplakin and other junction proteins provides a link between intercellular junctions and the actin and intermediate filament cytoskeleton. Maintenance and/or modification of this link is vital for control of cell adhesion and migration (1). γ-catenin is modified by phosphorylation, affecting both adhesion and β-catenin dependent transcription (7), and by and O-glycosylation, affecting adhesion (8). Recent evidence suggests that γ-catenin regulates desmosomal adhesion in response to growth factor stimulation (9). |
| 存放说明 | -20C |
| 参考文献 | Zhurinsky, J. et al. (2000) J Cell Sci 113 ( Pt 18), 3127-39. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. Zhurinsky, J. et al. (2000) Mol Cell Biol 20, 4238-52. Charpentier, E. et al. (2000) J Cell Biol 149, 503-20. Kolligs, F.T. et al. (2000) Genes Dev 14, 1319-31. Miravet, S. et al. (2003) Mol Cell Biol 23, 7391-402. Hu, P. et al. (2006) J Biol Chem 281, 12786-91. Yin, T. et al. (2005) J Biol Chem 280, 40355-63. |
Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® γ-Catenin siRNA I (Mouse Specific) (+) or SignalSilence® γ-Catenin siRNA II (Mouse Specific) #12680 (+), using γ-Catenin Antibody #2309 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The γ-Catenin Antibody confirms silencing of γ-catenin expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.Western blot分析转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)，SignalSilence® γ-Catenin siRNA I (Mouse Specific) (+)或者SignalSilence® γ-Catenin siRNA II (Mouse Specific) #12680 (+)后NIH/3T3细胞的提取物，采用的抗体是γ-Catenin Antibody #2309 (上图) 或β-Actin (D6A8) Rabbit mAb #8457 (下图)。γ-Catenin Antibody证实了γ-catenin的沉默表达，β-Actin (D6A8) Rabbit mAb为内参。 |
