pAM_1C Empty Vector
货号：
53023 基本售价：
3262.5 元 规格：
20 ug
产品信息
概述| 货号 | 53023 |
| 描述 | | Active Motifs Tag-ChIP-IT®Kit offers a method to perform ChIP without the need for protein-specific antibodies. Simply create an expression construct containing the protein of interest in-frame with a C-terminal AM-tag that is specifically designed to work in ChIP and produce highly consistent and reliable results. |
Active Motifs AM-tag offers several advantages over other tags, such as FLAG, GFP and HA. AM-tag is specifically designed to give low background signal in ChIP experiments. Also, in contrast to larger GFP and Halo® tags, the low molecular weight and unstructured design of the AM-tag reduces the possibility of impeding on the DNA binding domain. In addition, we have designed a high specificity antibody to the tag to increase the pull down efficiency during immunoprecipitation. Use the pAM_1C Empty Vector to clone your protein of interest, or add the AM-tag sequence to the expression vector of choice. Following transfection and expression of your tagged protein, the Tag-ChIP-IT Kit can be used to isolate chromatin and perform immunoprecipitations using an antibody directed against the AM-tag. The Tag-ChIP-IT method yields highly reproducible results with proven performance in both qPCR and ChIP-Seq analysis. Tag-ChIP-IT is Used to Identify Estrogen Receptor (ER) Binding Motifs.(Click image to enlarge) Tag-ChIP-IT®Advantages- Ideal for targets without ChIP antibodies or with low affinity antibodies
- Enables studies of isoforms, mutations and truncations
- AM-tag design maximizes enrichment efficiency of low abundance proteins
- AM-tag has little to no cross-reactivity with mammalian systems for reduced background
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性能| 供应商 | Active Motif |
| 运输条件 | Blue Ice |
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