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货号: bsm-33004M 基本售价: 580.0 元 规格: 50ul
- 规格:50ul
- 价格:580.00元
- 规格:100ul
- 价格:920.00元
- 规格:200ul
- 价格:1600.00元
产品信息
- 产品编号
- bsm-33004M
- 英文名称
- His tag
- 中文名称
- 聚组氨酸抗体标签单克隆抗体
- 别 名
- HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
- 规格价格
- 50ul/580元购买 100ul/920元购买 200ul/1600元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 免疫学
- 抗体来源
- Mouse
- 克隆类型
- Monoclonal
- 克 隆 号
- 6E6
- 交叉反应
- Human, fusion protein HHHHHH tagged
- 产品应用
- WB=1:1000-5000 IP=1:20-100 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated HHHHHH peptide:
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein G
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
SWISS:
N/A
Gene ID:
N/A
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白
1) 6xHis与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。
2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括:细菌,杆状病毒和哺乳动物)
4) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌。
5) 6xHis标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫。
- 产品图片
- Sample:
Lane1:Recombinant human JSRV-ENV Protein (Human) at 100 ng
Lane2:Recombinant human JSRV-ENV Protein (Human) at 100 ng
Primary: Anti-His tag (bsm-33004M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 35-45 kD
Observed band size: 40 kD