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货号： bsm-0954M 基本售价： 860.0 元 规格： 50ul
- 规格：50ul
- 价格：860.00元
- 规格：100ul
- 价格：1580.00元
产品信息
- 产品编号
- bsm-0954M
- 英文名称
- ET-1(2C4)
- 中文名称
- 内皮素-1单克隆抗体
- 别 名
- Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin1; ET 1; ET1; OTTHUMP00000039245; PPET1; Preproendothelin 1; Preproendothelin1
- 规格价格
- 50ul/860元购买 100ul/1580元购买 大包装/询价
- 说 明 书
- 50ul 100ul
- 研究领域
- 心血管 神经生物学
- 抗体来源
- Mouse
- 克隆类型
- Monoclonal
- 克 隆 号
- 2C4
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, Guinea Pig,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 2.5kDa
- 细胞定位
- 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human ET-1(CSCSSLMDKECVYFCHLDIIW Disulfide bridges C1-C15, C3-C11):53-73/202
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ETs can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
Function:
Endothelins are endothelium-derived vasoconstrictor peptides.
Subcellular Location:
Secreted.
Tissue Specificity:
Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
Similarity:
Belongs to the endothelin/sarafotoxin family.
SWISS:
P05305
Gene ID:
1906
Database links:Entrez Gene: 1906 Human
Entrez Gene: 13614 Mouse
Entrez Gene: 24323 Rat
Omim: 131240 Human
SwissProt: P05305 Human
SwissProt: P22387 Mouse
SwissProt: P22388 Rat
Unigene: 511899 Human
Unigene: 713645 Human
Unigene: 10918 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
内皮素(Endothelin-1,ET)是一种强的血管收缩剂，分为ET-1,ET-2,ET-3 三种,ET-1主要在内皮细胞中表达,而ET-2和ET-3则主要在神经系统中表达。ET具有广泛的生物学作用，此抗体是针对内皮素的活性片段（CSCSSLMDKECVYFCHLDIIW Disulfide bridges C1-C15, C3-C11）。
- 产品图片
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ET-1 Polyclonal Antibody, Unconjugated(bsm-0954M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining