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货号： bsm-33060M 基本售价： 860.0 元 规格： 50ul
- 规格：50ul
- 价格：860.00元
- 规格：100ul
- 价格：1580.00元
产品信息
- 产品编号
- bsm-33060M
- 英文名称
- CK7
- 中文名称
- 细胞角蛋白7单克隆抗体
- 别 名
- Cytokeratin 7; CK 7; K7; Keratin 7; Keratin,type II cytoskeletal 7; KRT7; SCL; K2C7_HUMAN.
- 规格价格
- 50ul/860元购买 100ul/1580元购买 大包装/询价
- 说 明 书
- 50ul 100ul
- 研究领域
- 肿瘤 信号转导
- 抗体来源
- Mouse
- 克隆类型
- Monoclonal
- 克 隆 号
- 10E3
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-1000 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 54kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CK7:
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein G
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Function:
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Subunit:
Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
Post-translational modifications:
Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P08729
Gene ID:
3855
Database links:Entrez Gene: 3855 Human
Omim: 148059 Human
SwissProt: P08729 Human
Unigene: 411501 Human
Unigene: 670221 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
结构蛋白（Structural Proteins）
CK-7是一种 54KDa 的中间丝蛋白，存在于大多数正常组织的腺上皮和移行上皮细胞中。 该抗体与多种良/恶性上皮性肿瘤反应。腺癌中的卵巢、乳腺、肺的腺癌呈阳性反应，而胃肠道的腺癌阴性。移行细胞肿瘤、前列腺癌也呈阳性反应。通常认为 CK7是腺癌和移行上皮细胞癌的比较特异性的标志。
- 产品图片
Sample:
Hela（human）Cell Lysate at 40 ug
MDA-MB-231（human） Cell Lysate at 40 ug
Primary: Anti-CK7 (bsm-33060M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Sample:A549(Human) Cell Lysate at 40 ug
Primary: Anti-CK7 (bsm-33060M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD