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货号： bsm-33377M 基本售价： 860.0 元 规格： 50ul
- 规格：50ul
- 价格：860.00元
- 规格：100ul
- 价格：1580.00元
产品信息
- 产品编号
- bsm-33377M
- 英文名称
- HP1 alpha
- 中文名称
- 异染色质蛋白1-α单克隆抗体
- 别 名
- Antigen p25; HP1-α; CBX5; CBX5_HUMAN; Chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox homolog 5; Chromobox protein homolog 5; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1 homolog alpha; HP1 alpha; HP1 alpha homolog; HP1; HP1A; HP1Hs alpha. HP-1-α; HP 1α; HP-1α; HP-1 α; HP1α; HP1 α;
- 规格价格
- 50ul/860元购买 100ul/1580元购买 大包装/询价
- 说 明 书
- 50ul 100ul
- 研究领域
- 免疫学 转录调节因子
- 抗体来源
- Mouse
- 克隆类型
- Monoclonal
- 克 隆 号
- 7E3
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:1000-5000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 22kDa
- 细胞定位
- 细胞核
- 性 状
- Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- Recombinant human HP1 alpha Protein:
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein G
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
HP1 alpha is a component of heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. HP1 alpha may interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane.
HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, the chromo shadow domain. Both domains of HP1 are requiredfor binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin.
Function:
Component of heterochromatin that recognizes and binds histone H3 tails methylated at Lys-9 (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when Tyr-41 of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Subunit:
Interacts with SUV420H1 and SUV420H2. Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at Lys-9. Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope. Interacts with CHAMP1. Interacts with ASXL1.
Subcellular Location:
Nucleus. Chromosome. Chromosome, centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
Post-translational modifications:
Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated.
Similarity:
Contains 2 chromo domains.
SWISS:
P45973
Gene ID:
23468
Database links:Entrez Gene: 538885 Cow
Entrez Gene: 477593 Dog
Entrez Gene: 23468 Human
Entrez Gene: 12419 Mouse
Entrez Gene: 300266 Rat
Omim: 604478 Human
SwissProt: P45973 Human
SwissProt: Q61686 Mouse
Unigene: 349283 Human
Unigene: 262059 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-HP1 alpha (bsm-33377M) at 1/2 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 24 kD
Sample:
Hela Cell (Human) Lysate at 40 ug
Primary: Anti-HP1 alpha (bsm-33377M) at 1/2 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 24 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (bsm-33377M 7E3) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.