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货号： bsm-33070M 基本售价： 860.0 元 规格： 50ul
- 规格：50ul
- 价格：860.00元
- 规格：100ul
- 价格：1580.00元
产品信息
- 产品编号
- bsm-33070M
- 英文名称
- Ki-67
- 中文名称
- Ki67蛋白单克隆抗体
- 别 名
- Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki-67; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN.
- 规格价格
- 50ul/860元购买 100ul/1580元购买 大包装/询价
- 说 明 书
- 50ul 100ul
- 研究领域
- 肿瘤 细胞生物 信号转导 转录调节因子
- 抗体来源
- Mouse
- 克隆类型
- Monoclonal
- 克 隆 号
- 6B9
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 358kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Ki-67:
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein G
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
Function:
Thought to be required for maintaining cell proliferation.
Subcellular Location:
Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
Similarity:
Contains 1 FHA domain.
SWISS:
P46013
Gene ID:
4288
Database links:
Entrez Gene: 4288 HumanEntrez Gene: 17345 Mouse
Entrez Gene: 246042 Rat
Omim: 176741 Human
SwissProt: P46013 Human
SwissProt: Q91VE6 Mouse
SwissProt: Q5RJM0 Rat
Unigene: 689823 Human
Unigene: 80976 Human
Unigene: 4078 Mouse
Unigene: 233802 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
细胞增殖标志物(Proliferation Marker)
Ki67与PCNA一样，为细胞增殖的一种标记，在细胞凋亡中S、G2 、M期均有表达，G0期缺如。 Ki-67增殖指数高低与许多肿瘤的分化程度、浸润、转移以及预后密切相关，因此被广泛作为各种恶性肿瘤的必检项目之一。
- 产品图片
Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Monoclonal Antibody, Unconjugated (bsm-33070M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Monoclonal Antibody, Unconjugated (bsm-33070M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Monoclonal Antibody, Unconjugated (bsm-33070M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.