Histone H4ac (pan-acetyl) antibody (pAb), sample【科瑞奥博】

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Histone H4ac (pan-acetyl) antibody (pAb), sample

货号： 39244 基本售价： 1435.5 元规格： 10 ul

产品信息

概述
货号	39244
同种亚型	Serum
反应种属	Human, Mouse, Other (Wide Range)
来源宿主	Rabbit
应用	ChIP/Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry
使用方法	Validated Applications: ChIP: 5 - 10 µl per ChIP ICC/IF: 1:500 - 1:2,000 dilution WB: 1:1,000 - 1:10,000 dilution Published Applications: see references

性能
供应商	Active Motif
纯化方式	None
免疫原	This Histone H4 pan-acetyl antibody was raised against a peptide containing the amino terminal region of Tetrahymena histone H2A acetylated at multiple lysines. (The immunogen has a high degree of homology to mammalian histone H4, so the antibody recognizes acetylated histone H4 in HeLa extracts).
背景	Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation.
运输条件	Blue Ice
存放说明	Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
存储溶液	Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an
计算分子量	8 kDa
参考文献

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参考图片

Histone H4 pan-acetyl antibody (pAb) tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT^® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10⁶ cell equivalents per ChIP) using 2 µl of Histone H4 pan-acetyl pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H4 pan-acetyl antibody (pAb) tested by immunofluorescence.
Staining of HeLa cells with Histone H4 pan-acetyl pAb (1:1,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).

Histone H4 pan-acetyl antibody (pAb) tested by Western blot.
HeLa acid extract (20 µg /lane) probed with Histone H4 pan-acetyl polyclonal antibody (1:10,000 dilution).
Lane 1: Cells treated with sodium butyrate.
Lane 2: No treatment.

Histone H4 pan-acetyl antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 pan-acetyl pAb for acetyl histone H4. Decreasing amounts of acetylated peptides corresponding to the immunogen, a related sequence, as well as unacetylated histone H4 peptides were spotted onto PVDF and probed with the antibody at a 1:10,000 dilution.
    Lane 1: AGG[acK]GG[acK]GMG[acK]VGA[acK]RHSC
    Lane 2: AGG[acK]GG[acK]GG[acK]GG[acK]GG[acK]GGC
    Lane 3: SGRGKGGKGLC
    Lane 4: GKGGAKRHRKC