| 货号 | 39150 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:10,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H3 phospho Ser28 antibody was raised against a peptide including phospho-serine 28 of histone H3. |
| 背景 | Histone H3 is one of five main histone proteins involved as core components of the nucleosome, the basic building block of chromatin. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Ser10 phosphorylation and Ser28 phosphorylation in the tail of H3 have very similar kinetics. Both phosphorylations occur early in mitosis when chromosomes begin to condense and during premature chromosome condensation induced in S-phase cells. These phosphorylated serines are excellent mitotic markers. In contrast to Ser10 phosphorylation, Ser28 phosphorylation has never been observed in interphase. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 phospho Ser28 pAb tested by Western blot. HeLa acid extract (5 µg per lane) was probed with Histone H3 phospho Ser28 pAb (1:5,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with colcemid. | |
Histone H3 phospho Ser28 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Ser28 pAb for phospho-Ser 10 of histone H3. Peptides corresponding to regions around major sites of histone H3 phosphorylation (serine 10 and serine 28) were spotted onto PVDF and probed with 39149 at a dilution of 1:5,000. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: Peptide phosphorylated at serine 10. Lane 2: Unmodified serine 10 peptide. Lane 3: Peptide phosphorylated at serine 28. Lane 4: Unmodified serine 28 peptide. |
