| 货号 | 55013 |
| 描述 | TheHydroxymethyl Collector™ Kits are designed to detect and capture DNA fragments containing 5-hydroxymethylcytosine (5-hmC) DNA methylation* for analysis. They utilize a β-glucosyltransferase enzyme to transfer a modified glucose moiety to 5-hydroxymethylcytosine residues in double-stranded DNA. This modified glucose is then used to chemically attach a biotin conjugate for capture and enrichment with streptavidin magnetic beads. TheHydroxymethyl Collector™ Kit utilizes between 5 ng and 2.5 μg of fragmented genomic DNA per capture reaction and is recommended for analysis of individual genes by PCR. The Hydroxymethyl Collector™–Seq Kit has a range of detection between 1 μg and 10 μg of fragmented genomic DNA per capture reaction and is optimized for use in combination with Next-Generation sequencing for genome-wide analysis of 5-hydroxymethylcytosine. Genome-wide analysis of 5-hmC in Mouse Brain, Kidney and Liver using Hydroxymethyl Collector–Seq.
By utilizing chemical labeling of 5-hmC residues, the Hydroxymethyl Collector Kits are extremely specific in their capture of hydroxymethylated DNA fragments. The biotin-streptavidin binding reaction also allows for stringent binding and wash conditions to reduce any non-specific background. The technique is sensitive enough to enrich DNA fragments containing two or more 5-hmC residues. Advantages of the Hydroxymethyl Collector Method for 5-hmC Enrichment
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| 供应商 | Active Motif |
| 运输条件 | Blue Ice |
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