MethylDetector™
货号：
55001 基本售价：
3045.0 元 规格：
50 rxns
产品信息
概述| 货号 | 55001 |
| 描述 | Active Motifs Bisulfite Conversion Kit simplifies the analysis of DNA methylation by providing optimized conversion reagents and an easy-to-use protocol. DNA of interest is rapidly heat denatured in a thermocycler in the presence of the bisulfite conversion reagent and a DNA denaturant. The conversion reaction can be performed in as little as 1.5 hours with a 99% conversion efficiency of unmethylated cytosines to uracils. After DNA conversion, the sample is added to the included DNA purification columns, and a simple, on-column desulfonation is performed. Ready-to-use DNA is then eluted from the columns. For your convenience, a positive control conversion-specific PCR primer pair that is specific for bisulfite converted human and mouse DNA is included. Because the primer pair produces a PCR product only if conversion has occurred, you can validate the success of the conversion reaction before spending extra time and money on sequencing. Active Motif also offers its original MethylDetector™ Bisulfite Modification Kit. This assay performs bisulfite conversion of DNA using the same principle as the Bisulfite Conversion Kit, but is optimized for use with a 5 hour conversion reaction. |
性能| 供应商 | Active Motif |
| 运输条件 | Blue Ice |
| 存放说明 | TheBisulfite Conversion Kit is shipped at room temperature and can be stored at 4°C prior to first use. Then, we recommend storing each component at the temperatures listed in the table below. Components are guaranteed stable for 6 months from the date of receipt when stored properly. This Bisulfite Conversion Kit includes the following components:- Conversion Reagent (5 tubes, lyophilized); Store at RT
- Denaturation Reagent; Store at RT
- Hydroquinone (5 tubes); Store at RT
- Buffer A; Store at RT
- Buffer B; Store at RT
- DNA Binding Buffer (2 bottles); Store at RT
- DNA Wash Buffer; Store at RT
- DNA Elution Buffer; Store at RT
- DNA purification columns and collection tubes (50 each); Store at RT
- Proteinase K (2 mg lyophilized); Store at -20°C
- Conversion-specific PCR primer pair (4 µM); Store at -20°C
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