NIH/3T3 whole-cell extract (TPA stimulated)
货号：
36415 基本售价：
2465.0 元 规格：
200 ug
产品信息
概述| 货号 | 36415 |
| 使用方法 | NIH/3T3 whole-cell extract (TPA stimulated) is specifically recommended for analysis of the mechanisms underlying tumorigenesis or TPA-mediated tumor conversion and its associated biochemical responses, including increased membrane metabolism, induction of protein biosynthesis, and alterations in the cell cycle. |
性能| 供应商 | Active Motif |
| 背景 | NIH/3T3 whole-cell extract (TPA stimulated) was prepared from a cell culture of the NIH/3T3 mouse fibroblast cell line obtained from Swiss mouse embryo tissue. This cell line was established from immortalized primary mouse embryonic fibroblast cells that were cultured by the 3T3 protocol. 3T3 (3-day transfer, inoculum 3 x 105 cells) refers to the transfer and inoculation protocol used to initiate and propagate the cell line. In research, these cells have become the standard mouse fibroblast cell line.
Treatment of NIH/3T3 cells with the potent tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) leads to various biological changes that mimic those observed in cells transformed by chemical carcinogens or viruses. In mice, TPA has been shown to induce long lasting effects, including inflammation and hyperplasia, that are associated with the critical first step in multistage tumorigenesis known as tumor conversion. Thus, TPA stimulated NIH/3T3 cells are frequently utilized in cancer research as a model system for studying the radiomimetic and tumor-promoting activity of TPA on replicating cells, particularly in relation to PKC-mediated growth control. |
| 运输条件 | Dry Ice |
| 存放说明 | To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles. |
| 外观 | 200 µg of NIH/3T3 whole-cell extract (TPA stimulated) at 5.0 µg/µl. |
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