| 货号 | AM-36111 |
| 使用方法 | HeLa nuclear extract (Anisomycin treated) is specifically recommended for analysis of protein synthesis and the p38/JNK MAPK pathway. |
| 供应商 | Active Motif |
| 背景 | HeLa nuclear extract (Anisomycin treated) was prepared from a cell culture of the HeLa human epithelial carcinoma cell line that was treated with anisomycin, an antibiotic and protein synthesis inhibitor. Anisomycin is also a potent inhibitor of the p38/JNK MAPK pathway. The HeLa cell line was originally derived from cells obtained in 1951 from Henrietta Lacks, a cervical cancer patient who eventually died from her cancer. The HeLa cell line is one of the oldest and most commonly utilized immortal cell lines in scientific research. These cells are highly prolific and easily sustainable, allowing for flexibility in their application in various fields of scientific research, including cancer biology, virology, and human disease. To date, over 60,000 publications across various disciplines have referenced the use of HeLa cells. |
| 运输条件 | Dry Ice |
| 存放说明 | To ensure stability, extracts should be stored at -80°C. We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles. |
| 外观 | 2 x 100 µg of HeLa nuclear extract (Anisomycin treated) at 2.5 µg/µl. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Figure 1: Monitoring ATF-2 activation with the TransAM ATF-2 Kit. Increasing amounts of nuclear extract from unstimulated HeLa and Anisomycin-stimulated HeLa cells are tested for ATF-2 activation using the TransAM ATF-2 Kit. |
