K-562 nuclear extract (TPA stimulated)
货号：
36070 基本售价：
2465.0 元 规格：
200 ug
产品信息
概述| 货号 | 36070 |
| 使用方法 | K-562 nuclear extract is recommended for use in studies related to 1) differentiation, 2) immunology, and 3) cancer. K-562 nuclear extract (TPA stimulated) is specifically recommended for analysis of the mechanisms underlying tumorigenesis or TPA-mediated tumor conversion and its associated biochemical responses, including increased membrane metabolism, induction of protein biosynthesis, and alterations in the cell cycle. |
性能| 供应商 | Active Motif |
| 背景 | K-562 nuclear extract (TPA stimulated) was prepared from a cell culture of the K-562 human myelogenous leukaemia cell line. The K-562 cell line was originally derived from immortalized erythroleukaemia cells obtained from a patient with a Philadelphia chromosome-positive chronic myeloid leukemia in blast crisis. These pluripotent cells are widely applicable in research due to their ability to spontaneously differentiate into erythrocyte, granulocyte and monocyte progenitors. K-562 cells are also frequently utilized in in vitro natural killer (NK) cell assays as highly sensitive targets since they lack the expression of MHC class I molecules required for protection against NK activity.
Treatment of K-562 cells with the potent tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) leads to various biological changes that mimic those observed in cells transformed by chemical carcinogens or viruses. Tumor promoters such as TPA are thought to play a significant role in the critical first step in multistage tumorigenesis known as tumor conversion. Treatment of K-562 cells with TPA leads to induction of megakaryocytic differentiation and decreased susceptibility to NK activity. Thus, TPA stimulated K-562 cells are frequently utilized in cancer research as a model system for studying the radiomimetic and tumor-promoting effects of TPA on the differentiation stage of human malignant cells. |
| 运输条件 | Dry Ice |
| 存放说明 | To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles. |
| 外观 | 2 x 100 µg of K-562 nuclear extract (TPA stimulated) at 2.5 µg/µl. |
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