293 nuclear extract
货号：
36033 基本售价：
2465.0 元 规格：
200 ug
产品信息
概述| 货号 | 36033 |
| 使用方法 | 293 nuclear extract is specifically recommended for analysis of 1) in vitrotranscription, 2) protein-protein and protein-DNA/RNA interactions, and 3) in vitrosplicing. |
性能| 供应商 | Active Motif |
| 背景 | 293 nuclear extract was prepared from cell cultures of the Human Embryonic Kidney (HEK) 293 cell line. This cell line was originally derived from transformation of normal human embryonic tissue cultures with a sheared DNA fragment of the left arm of the adenoviral 5 viral genome. These cells are commonly used in research due to their ease of growth and transfectability. However, since the cellular origin of these hypotriploid cells is poorly understood, they are not recommended for use as an in vitro model for kidney cell function. 293 nuclear extract contains all the basal transcriptional and mRNA splicing components and serves as an ideal cell-free platform for analysis of transcription and pre-mRNA processing mechanisms. |
| 运输条件 | Dry Ice |
| 存放说明 | To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles. |
| 外观 | 200 µg of 293 nuclear extract at 4.68 µg/µl. |
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